Advanced Biochemistry Lab Syllabus

Spring 2023
TR 11-1:50


Dr. Koni Stone, ude.natsusc|enotsk#ude.natsusc|enotsk, Office hours: M 9-10, 2-3, T 10-11, R 10-11, F 11-12
Dr. Gönül Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg, Office hours: MWF 12:00-1:00pm and by appointment
When sending electronic mail, please put BiochemLab in the subject line, for speedy assistance. When leaving a voice mail message, be sure to clearly state your phone number.

Materials for each lab will be posted, no text is assigned.

Learning Goals

Students will be able to write organized lab reports.
Students will use information and procedures from published sources for biochemical experiments.
Students will function effectively in teams.
Students will understand and apply scientific methods of experimental design and analysis.

Assessement Device total points Dates
Class activities 100 every lab, be on time, be prepared and record everything in your notebook
Make a buffer 100 Feb 22
Protein Database Lab 100 March 2
Microsome assay (in person) 100 May 16
Protein 1: Protein engineering 1 100 See Canvas
Protein 2: Protein engineering 1 100 See Canvas
Protein 3: Protein engineering 1 100 See Canvas
Final Poster Presentation of an Experiment 100 Thursday, May 18, 11:15

Grades are assigned with the following minimums: A= 90%, B= 80%, C= 70%, D=60%

Class activities:
Be on time. The points you earn will be converted to a percentage of the total points. There are no make-up in class points.

Normal laboratory safety rules will be followed. These include:

  • Appropriate eye protection must be worn whenever working in the lab.
  • Appropriate clothing and closed toe shoes must be worn in the lab.
  • No smoking, eating, or drinking in the lab.
  • Please familiarize yourself with the location of the safety equipment in the laboratory.
  • You must complete your work during your regularly scheduled lab period. You may be approved to use an instrument outside of lab time. If you request access to an instrument outside of your lab time, your lab instructor must approve it, and a faculty member must be available to supervise you.


The list of experiments for the laboratory portion of the course has a brief description of each experiment and links to pages with more details. Please consult the lab schedule for the start dates and due dates for each experimental report.

You must properly plan your experiments before coming to the laboratory. Simply reading the procedure will not be enough preparation. Carefully read the experiment and plan your laboratory work in advance to make the best use of the time available. Advance planning will allow you to begin procedures that require large blocks of time at the beginning of the laboratory period. Also, you must be cleared to use an instrument BEFORE you can begin working on it.  Each student must be checked out on an instrument by the instructor before using it.  Consult with the instructor before starting an experiment.

A permanently bound notebook will be provided and must be used. You should consecutively number each page and include a table of contents.  All data, observations, and conclusions must be recorded directly into the notebook in ink.  Do not record raw data on scraps of paper. All work must be dated, and signed by an instructor each day you are in lab. Lab notebooks will be turned in at the end of the semester. Your notebook record should include:

Purpose: Identify the fundamental scientific reason(s) the experiment is being conducted, and the general type of data that will be collected (instrumentation, components, design, etc.) to address the purpose.
Experimental Section: This section should describe how you obtained the data (and not how you manipulated or interpreted the data).  Describe the experimental method that you used, including any unusual circumstances. Anyone following your experimental section should be able to repeat your experiment precisely.  It will not suffice to "refer to the hand-out" nor should you copy the hand-out. Use your own words, as there is zero tolerance of plagiarism.  Include sample calculations for solution concentrations or other related calculations as well as mass and mole values of quantities used.  Report information on instruments used and the source of chemical reagents.  Note any warnings from material safety data sheets (MSDS) on particularly dangerous chemicals and attach MSDS copies as an appendix to your report.
Data: Include all data collected in lab, written in ink, in the notebook. The exceptions are spectra and chromatograms. You may paste those into your notebook in the Results section. You must have your data signed by your instructor on the day that it was collected.
Data analysis: Show all sample calculations, including statistical analysis of your data. Clearly explain what you are calculating and why. This section should be neat, organized and succinct.

Results and Discussion: This section will include your outcomes, expected and unexpected.  Calculations of any result from your collected data should be reported here.  All results must be introduced in the text and displayed in well organized tables and graphs. Each table and graph should be explained in the text, tell what it is, how you got the results and explain the significance of these results. Explain your results to the reader. Error analysis is important in determining how significant your results are. When appropriate,use error analysis to report the 95% confidence range of your unknown determination; also, use error analysis to determine how many significant figures to report.

Conclusion: Summarize your fundamental results and how they relate to the expected (literature) results. Be sure to include the uncertainty of your final data and report your data with the correct number of significant figures. Also, you must state any unknown numbers in the conclusion. 
References: Include all sources cited and use the ACS style for citing references.

Labs are due on the dates specified on the list of experiments. Late labs are penalized 10% per day that they are late. (Do not be late.) Submit your lab reports to

Make A Buffer:
More details are available on the Make a Buffer page. You will turn in a report of the project to, on or before the due date. Late projects will be penalized 10% for every day they are late. 

Note: Do not expect extensive help the last days before a report or lab is due.  Complete the reports in a timely fashion as expected of all professionals. 

Poster Presentations:
Each student will give a poster presentation in their lab period time during finals week. Your instructors will be inviting other professionals from our academic community.  Expectations for the presentations will be discussed in class and the rubric is posted on the schedule page. 

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