Biochemistry Lab
CHEM 4422 Advanced Biochemistry Lab at CSU, Stanislaus
Spring 2021
TR 11-1:50
Instructors: Dr. Koni Stone and Dr. Gönül Schara
Email:
Dr. Stone, moc.em|enotskk#moc.em|enotskk
Dr. Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg
Turnintin: Details will be provided by email
Syllabus:
Updated schedule of events:
| Date | Description | where/what? |
|---|---|---|
| Feb 22 | Buffer Lab report | Turnitin to Dr. Stone |
| March 2 | Protein models | Presentation of your protein model to the class, turnitin to Dr. Stone |
| April 12 | Microsome lab | Sign up for in person pipetting, its optional unless you want to do the microsome lab. The sign up is a google doc, sent by email. |
Literature Search
Instructions for searching Journal Data bases, including ACS Publications, Sci Finder and Pub Med
List of Experiments
Details will be linked to these topics.| Start Date | Experiment | Points | Report due date |
|---|---|---|---|
| Jan 28 | Protein Database Lab You will be viewing an important part of the enzyme Cytidine Deaminase (5keg.pdb) Here is the rubric. Also, the rubric is on the Turnitin page for this assignment. | 100 | March 2, Oral presentation of your structure in class, report to turnitin by 11:59pm |
| Jan 28 | Buffers and here is the rubric | 100 | Feb 22 |
| April 12 | Optional: In person pipetting, sign up on the google doc that you got via email. Microsome Assay and here is some basic information Microsome lab report instructions/or Find your Yeast | 100 | May 13 |
| Feb 18 | Three Protein Engineering Labs (See Dr. Schara's blackboard page for more information | 300 | TBD |
| May 20 11:15 | Final Poster Presentation of an Experiment from CHEM 4422, here is the Rubric for the Poster Presentation (multiply each category by 2) | 100 each | May 20, 11:15-1:15 poster presentation |
Interesting sites on the web:
PDB resources
Introduction to Practical Biochemisry] an e-text book. You will want to consult this often.
http://cariboubio.com This company uses a protein (Cas9) that can selectively edit DNA. This protein is a CRISPR(clustered regularly interspaced short palindromic repeats) binding protein—proteins that are part of the prokaryotic immune system. The CEO, Rachel Haurwitz, gave a talk at the CSUPERB symposium in January 2015.
