Biochemistry Lab
CHEM 4422 Advanced Biochemistry Lab at CSU, Stanislaus
Spring 2019
TR 11-1:50
Instructors: Dr. Koni Stone and Dr. Gönül Schara
Email:
Dr. Stone, moc.em|enotskk#moc.em|enotskk
Dr. Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg
Turnintin:
Syllabus:
Updated schedule of events:
| Date | Description | where/what? |
| April 7 | Protein Lab 1 | submit to Dr. Schara via Blackboard by 11 am |
| April 7 | Protein Lab 3: Find your Yeast | Zoom conference at 11am |
| April 9 | Protein Lab 2: Autodoc for All! | Zoom conference at 11am |
| April 17 | Microsome Lab | Submit for feedback to Dr. Stone's turnitin.com |
Literature Search
Instructions for searching Journal Data bases, including ACS Publications, Sci Finder and Pub Med
List of Experiments
Details will be linked to these topics.| Start Date | Experiment | Points | Report due date |
|---|---|---|---|
| Jan 28 | Protein Database Lab You will be viewing Rabbit Muscle Creatine Kinase (2CRK.PDB) Here is the rubric, ignore the request for the structure on Friday, it is due Monday by 5pm. Also, the rubric is on the Turnitin page for this assignment. | 100 | March 2, structure by 5pm, report to turnitin by 11:59pm |
| Jan 28 | Buffers and here is the rubric | 100 | Feb 21 |
| Feb 11 | Microsome Assay and here is some basic informationMicrosome lab report instructions | 100 | |
| Feb 18 | Three Protein Engineering Labs (See Dr. Schara's blackboard page for more information | 300 | TBD |
| May 19 11:15 | Final Poster Presentation of an Experiment from CHEM 4422, here is the Rubric for the Poster Presentation (multiply each category by 2) | 100 each | May 19, 11:15-1:15 poster presentation |
Interesting sites on the web:
PDB resources
Introduction to Practical Biochemisry] an e-text book. You will want to consult this often.
http://cariboubio.com This company uses a protein (Cas9) that can selectively edit DNA. This protein is a CRISPR(clustered regularly interspaced short palindromic repeats) binding protein—proteins that are part of the prokaryotic immune system. The CEO, Rachel Haurwitz, gave a talk at the CSUPERB symposium in January 2015.
