Biochemistry Lab
CHEM 4422 Advanced Biochemistry Lab at CSU, Stanislaus
Spring 2022
TR 11-1:50
Instructors: Dr. Koni Stone and Dr. Gönül Schara
Email:
Dr. Stone, moc.em|enotskk#moc.em|enotskk
Dr. Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg
Turnintin: Details will be provided by email
Syllabus:
Updated schedule of events:
| Date | Description | where/what? |
|---|---|---|
| Feb 22 | Buffer Lab report | Submit to Turnitin to Dr. Stone |
| March 3 April 5 | Protein models | Bring your model to the class, submit report by midnight to Turnitin.com |
| April 12 | Microsome lab | Submit report to Turnitin.com |
Literature Search
Instructions for searching Journal Data bases, including ACS Publications, Sci Finder and Pub Med
List of Experiments
Details will be linked to these topics.| Start Date | Experiment | Points | Report due date |
|---|---|---|---|
| Jan 28 | Protein Database Lab You will be viewing an important part of a protein that inhibits Crisper CAS-9rubric. Also, the rubric is on the Turnitin page for this assignment. | 100 | March 3, April 5 bring your structure to class, submit report to Turnitin by 11:59pm |
| Jan 28 | Buffers and here is the rubric | 100 | Feb 22 |
| April 12 | Microsome Assay and here is some basic information Microsome lab report instructions/ | 100 | May 15 |
| Feb 17 | Three Protein Engineering Labs (See Dr. Schara's Canvas page for more information | 300 | TBD |
| May 19 11:15 | Final Poster Presentation of an Experiment from CHEM 4422, here is the Rubric for the Poster Presentation (multiply each category by 2) | 100 each | May 19, 11:15-1:15 poster presentation |
Interesting sites on the web:
PDB resources
Introduction to Practical Biochemisry] an e-text book. You will want to consult this often.
http://cariboubio.com This company uses a protein (Cas9) that can selectively edit DNA. This protein is a CRISPR(clustered regularly interspaced short palindromic repeats) binding protein—proteins that are part of the prokaryotic immune system. The CEO, Rachel Haurwitz, gave a talk at the CSUPERB symposium in January 2015.
