Biochemistry Lab

CHEM 4422 Advanced Biochemistry Lab at CSU, Stanislaus

Spring 2022

TR 11-1:50

Instructors: Dr. Koni Stone and Dr. Gönül Schara

Dr. Stone, moc.em|enotskk#moc.em|enotskk
Dr. Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg

Turnintin: Details will be provided by email



Updated schedule of events:

Date Description where/what?
Feb 22 Buffer Lab report Submit to Turnitin to Dr. Stone
March 3 April 5 Protein models Bring your model to the class, submit report by midnight to
April 12 Microsome lab Submit report to

Literature Search

Instructions for searching Journal Data bases, including ACS Publications, Sci Finder and Pub Med

List of Experiments

Details will be linked to these topics.
Start Date Experiment Points Report due date
Jan 28 Protein Database Lab You will be viewing an important part of a protein that inhibits Crisper CAS-9rubric. Also, the rubric is on the Turnitin page for this assignment. 100 March 3, April 5 bring your structure to class, submit report to Turnitin by 11:59pm
Jan 28 Buffers and here is the rubric 100 Feb 22
April 12 Microsome Assay and here is some basic information Microsome lab report instructions/ 100 May 15
Feb 17 Three Protein Engineering Labs (See Dr. Schara's Canvas page for more information 300 TBD
May 19 11:15 Final Poster Presentation of an Experiment from CHEM 4422, here is the Rubric for the Poster Presentation (multiply each category by 2) 100 each May 19, 11:15-1:15 poster presentation

Interesting sites on the web:

PDB resources
Introduction to Practical Biochemisry] an e-text book. You will want to consult this often. This company uses a protein (Cas9) that can selectively edit DNA. This protein is a CRISPR(clustered regularly interspaced short palindromic repeats) binding protein—proteins that are part of the prokaryotic immune system. The CEO, Rachel Haurwitz, gave a talk at the CSUPERB symposium in January 2015.

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