Biochemistry Lab

CHEM 4422 Advanced Biochemistry Lab at CSU, Stanislaus

Spring 2021

TR 11-1:50

Instructors: Dr. Koni Stone and Dr. Gönül Schara

Dr. Stone, moc.em|enotskk#moc.em|enotskk
Dr. Schara, ude.natsusc|arahcsg#ude.natsusc|arahcsg

Turnintin: Details will be provided by email



Updated schedule of events:

Date Description where/what?
Feb 22 Buffer Lab report Turnitin to Dr. Stone
March 2 Protein models Presentation of your protein model to the class, turnitin to Dr. Stone
April 12 Microsome lab Sign up for in person pipetting, its optional unless you want to do the microsome lab. The sign up is a google doc, sent by email.

Literature Search

Instructions for searching Journal Data bases, including ACS Publications, Sci Finder and Pub Med

List of Experiments

Details will be linked to these topics.
Start Date Experiment Points Report due date
Jan 28 Protein Database Lab You will be viewing an important part of the enzyme Cytidine Deaminase (5keg.pdb) Here is the rubric. Also, the rubric is on the Turnitin page for this assignment. 100 March 2, Oral presentation of your structure in class, report to turnitin by 11:59pm
Jan 28 Buffers and here is the rubric 100 Feb 22
April 12 Optional: In person pipetting, sign up on the google doc that you got via email. Microsome Assay and here is some basic information Microsome lab report instructions/or Find your Yeast 100 May 13
Feb 18 Three Protein Engineering Labs (See Dr. Schara's blackboard page for more information 300 TBD
May 20 11:15 Final Poster Presentation of an Experiment from CHEM 4422, here is the Rubric for the Poster Presentation (multiply each category by 2) 100 each May 20, 11:15-1:15 poster presentation

Interesting sites on the web:

PDB resources
Introduction to Practical Biochemisry] an e-text book. You will want to consult this often. This company uses a protein (Cas9) that can selectively edit DNA. This protein is a CRISPR(clustered regularly interspaced short palindromic repeats) binding protein—proteins that are part of the prokaryotic immune system. The CEO, Rachel Haurwitz, gave a talk at the CSUPERB symposium in January 2015.

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